alexa 546 conjugated anti cd8  (Vector Laboratories)

Journal: International Journal of Molecular Sciences

Article Title: Pathological Analysis of Ocular Lesions in a Murine Model of Sjögren’s Syndrome

doi: 10.3390/ijms18061209

Figure Lengend Snippet: Immune cell infiltration of target organs of the SS model mice. CD4, CD8, CD19, and F4/80 expressing immune cells were observed by confocal microscopy. ( a ) CD4 + T cells, ( b ) CD8 + T cells, ( c ) CD19 + B cells, and ( d ) F4/80 + macrophages in the lacrimal glands of the SS model mice were detected by Alexa-546-conjugated antibodies. Nuclei were stained with 4′,6-diamdino-2-phenylindole dihydrochloride (DAPI). The results are representative of five mice. Scale bar: 50 μm; ( e ) Flow cytometry analysis using lacrimal gland tissues from the SS model mice. CD4 and CD8 T cell subsets ware shown, and the result is representative of 5 mice; and ( f ) Cell numbers of CD4 + , CD8 + T cells, CD19 + B cells, and F4/8 + macrophages were calculated by flow cytometery analysis. Data are means ± SD of 5 mice.

Article Snippet: Frozen sections of lacrimal gland tissues were fixed in 4% paraformaldehyde in phosphate buffer, blocked with Blocking One Histo (Nacalai Tesque, Kyoto, Japan), and then stained with Alexa 546-conjugated rat anti-mouse CD4, CD8, CD19, or F4/80 monoclonal antibodies (eBiosciences, San Diego, CA, USA) overnight.

Techniques: Expressing, Confocal Microscopy, Staining, Flow Cytometry